If your Folder/Files Disappear from flash driver ( USB DRIVE ) & Showing Desktop.ini file. Use Below Method.

Deleting shortcut making virus and desktop.ini that opens at startup

To Delete shortcut making virus :--->

After plug in your virus affected drive, just press Win key + R to open Run box and type “cmd” and press Enter key. (Without double quotes)

In the opened command prompt, open your drive by enter your drive letter.

For example, if your drive letter is enter g: and press Enter. (To find your drive letter just open “My Computer” and check your drive icon)

Now type “del *.lnk” and hit Enter.(without double quotes)

Then type the below command and hit Enter. (without double quotes)
“attrib -s -r -h *.* /s /d /l”

That’s it, just wait some seconds. Then open and check your drive. Your folders will be retrieved